NILE CHEMICALS

TRIS   BUFFER  GR

SPECIFICATION

NH2C(CH2OH)3   M.W.  121.14  
Minimum assay ( HCIO4 ) titration:  
on anhydrous substances 99.5%
IR Spectrum passes test
pH ( 5% water ) 10.0 - 11.0
Absorptivity A 250 - 440 nm  
( 1cm; 10% water ) passes test
Melting point 169 - 171OC
Maximum Limits of Impurities :  
Chloride ( CI ) 0.001%
Sulphated ash 0.01%
Water 0.5%
Biological buffer  
( Standard titrimetric substance  
Specially prepared for biochemistry and  
enzymology pKa - 8.07 at 25OC )  

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